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Descriptive title : An assay for pyridoxine in food and feeds.
Corporate Author : NIJ (Publication Date)
Personal Author(s) : Ananthan,Karim S.
Report Date : 1988
Pagination or Media Count : 38
Abstract :
PURPOSE. The purpose of this method is to establish a method of determining the concentration of vitamin B6 in foods. BACKGROUND. The literature has suggested that the current methods available for determining the concentration of pyridoxal-5′-phosphate (PLP), an essential form of vitamin B6, in foods are inadequate. 1. METHODS. The working assay is based on the ability of PLP to transfer phosphate from ATP to pyridoxal-5′-phosphate dehydrogenase. 2. A simple, one-step procedure is described. It involves the formation of a complex between a group from the food sample and alkaline phosphatase. The bound group is used for the determination of PLP by monitoring the release of phosphate from the enzyme-PLP complex on addition of the cofactor pyridoxal-5′-phosphate dehydrogenase. 3. The method is applicable to both micro-nutrient and non-nutrient containing foods and has been successfully demonstrated on a wide range of food samples. 4. Use of the method will allow laboratories to meet the needs of their clients for reliable, accurate vitamin B6 assay information in less than 10 min. 5. Practical applications of the method will
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